READ THE MANUAL and do several test runs using water so you know what to expect..
1. Fill the fluid bag with approximately 75 to 85 ml of warm water or warm clean urine.
2. Remove as much air from the bag as possible and screw cap back on the end of the tube.
3. Attach your two 9-volt Duracell batteries and fold fabric shut, making sure to secure the ends.
4. Once your temperature indicator on the bag is reading 98 to 100 you are ready to give your sample.